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ku80 polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech ku80 polyclonal antibody
    Ku80 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ku80+polyclonal+antibody/XRCC5%2FKu80+Antibody/pm41535889-236-53-56
    Average 94 stars, based on 47 article reviews
    ku80 polyclonal antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Gentle:

    Article Title: Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway
    Article Snippet: Total cellular proteins were extracted and separated by 10% ExpressCast PAGE Gel preparation kit (#P2012, New Cell & Molecular Biotech., Suzhou, China). .. Separated protein bands were transferred to polyvinylidene fluoride (PVDF) membranes (#IPVH00010, Millipore, Boston, USA), which were then blocked with skim milk (#VIC141, VICMED, Xuzhou, China) by gentle shaking at room temperature for 1 h. The PVDF membranes were incubated overnight at 4 °C with the following primary antibodies: MSN polyclonal antibody (1:1000; #16495-1-AP, Proteintech, Wuhan, China), CD44 polyclonal antibody (recognizes the standard isoform of human CD44, 1:1000; #15675-1-AP, Proteintech, Wuhan, China), DNA-PKcs polyclonal antibody (1:1000; #19983-1-AP, Proteintech, Wuhan, China), KU70 polyclonal antibody (1:1000; #10723-1-AP, Proteintech, Wuhan, China), KU80 polyclonal antibody (1:1000; #16389-1-AP, Proteintech, Wuhan, China), AKT1 monoclonal antibody(2C5D1) (1:1000; #60203-2-Ig, Proteintech, Wuhan, China), phospho-AKT1 (Ser473) recombinant antibody(2E17) (1:1000; #80455-1-RR, Proteintech, Wuhan, China), and GAPDH monoclonal antibody(1E6D9) (1:1000; #60004-1-Ig, Proteintech, Wuhan, China). .. 12 h later, the PVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:10000; #VSE12 and #VSE13, VICMED, Xuzhou, China) at room temperature for 1 h. After washing with washing buffer for 3 times, protein bands were visualized using an ECL chemiluminescence kit (# P10300 , New Cell & Molecular Biotech., Suzhou, China) and a chemiluminescence imaging system (Tanon, Shanghai, China).

    Article Title: Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway.
    Article Snippet: Total cellular proteins were extracted and separated by 10% ExpressCast PAGE Gel preparation kit (#P2012, New Cell & Molecular Biotech., Suzhou, China). .. Separated protein bands were transferred to polyvinylidene fluoride (PVDF) membranes (#IPVH00010, Millipore, Boston, USA), which were then blocked with skim milk (#VIC141, VICMED, Xuzhou, China) by gentle shaking at room temperature for 1 h. The PVDF membranes were incubated overnight at 4 °C with the following primary antibodies: MSN polyclonal antibody (1:1000; #16495-1-AP, Proteintech, Wuhan, China), CD44 polyclonal antibody (recognizes the standard isoform of human CD44, 1:1000; #15675-1-AP, Proteintech, Wuhan, China), DNA-PKcs polyclonal antibody (1:1000; #19983-1-AP, Proteintech, Wuhan, China), KU70 polyclonal antibody (1:1000; #10723-1-AP, Proteintech, Wuhan, China), KU80 polyclonal antibody (1:1000; #16389-1-AP, Proteintech, Wuhan, China), AKT1 monoclonal antibody(2C5D1) (1:1000; #60203-2-Ig, Proteintech, Wuhan, China), phospho-AKT1 (Ser473) recombinant antibody(2E17) (1:1000; #80455-1-RR, Proteintech, Wuhan, China), and GAPDH monoclonal antibody(1E6D9) (1:1000; #60004-1- Ig, Proteintech, Wuhan, China). .. 12 h later, the PVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:10000; #VSE12 and #VSE13, VICMED, Xuzhou, China) at room temperature for 1 h. After washing with washing buffer for 3 times, protein bands were visualized using an ECL chemiluminescence kit (#P10300, New Cell & Molecular Biotech., Suzhou, China) and a chemiluminescence imaging system (Tanon, Shanghai, China).

    Incubation:

    Article Title: Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway
    Article Snippet: Total cellular proteins were extracted and separated by 10% ExpressCast PAGE Gel preparation kit (#P2012, New Cell & Molecular Biotech., Suzhou, China). .. Separated protein bands were transferred to polyvinylidene fluoride (PVDF) membranes (#IPVH00010, Millipore, Boston, USA), which were then blocked with skim milk (#VIC141, VICMED, Xuzhou, China) by gentle shaking at room temperature for 1 h. The PVDF membranes were incubated overnight at 4 °C with the following primary antibodies: MSN polyclonal antibody (1:1000; #16495-1-AP, Proteintech, Wuhan, China), CD44 polyclonal antibody (recognizes the standard isoform of human CD44, 1:1000; #15675-1-AP, Proteintech, Wuhan, China), DNA-PKcs polyclonal antibody (1:1000; #19983-1-AP, Proteintech, Wuhan, China), KU70 polyclonal antibody (1:1000; #10723-1-AP, Proteintech, Wuhan, China), KU80 polyclonal antibody (1:1000; #16389-1-AP, Proteintech, Wuhan, China), AKT1 monoclonal antibody(2C5D1) (1:1000; #60203-2-Ig, Proteintech, Wuhan, China), phospho-AKT1 (Ser473) recombinant antibody(2E17) (1:1000; #80455-1-RR, Proteintech, Wuhan, China), and GAPDH monoclonal antibody(1E6D9) (1:1000; #60004-1-Ig, Proteintech, Wuhan, China). .. 12 h later, the PVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:10000; #VSE12 and #VSE13, VICMED, Xuzhou, China) at room temperature for 1 h. After washing with washing buffer for 3 times, protein bands were visualized using an ECL chemiluminescence kit (# P10300 , New Cell & Molecular Biotech., Suzhou, China) and a chemiluminescence imaging system (Tanon, Shanghai, China).

    Article Title: Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway.
    Article Snippet: Total cellular proteins were extracted and separated by 10% ExpressCast PAGE Gel preparation kit (#P2012, New Cell & Molecular Biotech., Suzhou, China). .. Separated protein bands were transferred to polyvinylidene fluoride (PVDF) membranes (#IPVH00010, Millipore, Boston, USA), which were then blocked with skim milk (#VIC141, VICMED, Xuzhou, China) by gentle shaking at room temperature for 1 h. The PVDF membranes were incubated overnight at 4 °C with the following primary antibodies: MSN polyclonal antibody (1:1000; #16495-1-AP, Proteintech, Wuhan, China), CD44 polyclonal antibody (recognizes the standard isoform of human CD44, 1:1000; #15675-1-AP, Proteintech, Wuhan, China), DNA-PKcs polyclonal antibody (1:1000; #19983-1-AP, Proteintech, Wuhan, China), KU70 polyclonal antibody (1:1000; #10723-1-AP, Proteintech, Wuhan, China), KU80 polyclonal antibody (1:1000; #16389-1-AP, Proteintech, Wuhan, China), AKT1 monoclonal antibody(2C5D1) (1:1000; #60203-2-Ig, Proteintech, Wuhan, China), phospho-AKT1 (Ser473) recombinant antibody(2E17) (1:1000; #80455-1-RR, Proteintech, Wuhan, China), and GAPDH monoclonal antibody(1E6D9) (1:1000; #60004-1- Ig, Proteintech, Wuhan, China). .. 12 h later, the PVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:10000; #VSE12 and #VSE13, VICMED, Xuzhou, China) at room temperature for 1 h. After washing with washing buffer for 3 times, protein bands were visualized using an ECL chemiluminescence kit (#P10300, New Cell & Molecular Biotech., Suzhou, China) and a chemiluminescence imaging system (Tanon, Shanghai, China).

    Recombinant:

    Article Title: Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway
    Article Snippet: Total cellular proteins were extracted and separated by 10% ExpressCast PAGE Gel preparation kit (#P2012, New Cell & Molecular Biotech., Suzhou, China). .. Separated protein bands were transferred to polyvinylidene fluoride (PVDF) membranes (#IPVH00010, Millipore, Boston, USA), which were then blocked with skim milk (#VIC141, VICMED, Xuzhou, China) by gentle shaking at room temperature for 1 h. The PVDF membranes were incubated overnight at 4 °C with the following primary antibodies: MSN polyclonal antibody (1:1000; #16495-1-AP, Proteintech, Wuhan, China), CD44 polyclonal antibody (recognizes the standard isoform of human CD44, 1:1000; #15675-1-AP, Proteintech, Wuhan, China), DNA-PKcs polyclonal antibody (1:1000; #19983-1-AP, Proteintech, Wuhan, China), KU70 polyclonal antibody (1:1000; #10723-1-AP, Proteintech, Wuhan, China), KU80 polyclonal antibody (1:1000; #16389-1-AP, Proteintech, Wuhan, China), AKT1 monoclonal antibody(2C5D1) (1:1000; #60203-2-Ig, Proteintech, Wuhan, China), phospho-AKT1 (Ser473) recombinant antibody(2E17) (1:1000; #80455-1-RR, Proteintech, Wuhan, China), and GAPDH monoclonal antibody(1E6D9) (1:1000; #60004-1-Ig, Proteintech, Wuhan, China). .. 12 h later, the PVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:10000; #VSE12 and #VSE13, VICMED, Xuzhou, China) at room temperature for 1 h. After washing with washing buffer for 3 times, protein bands were visualized using an ECL chemiluminescence kit (# P10300 , New Cell & Molecular Biotech., Suzhou, China) and a chemiluminescence imaging system (Tanon, Shanghai, China).

    Article Title: Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway.
    Article Snippet: Total cellular proteins were extracted and separated by 10% ExpressCast PAGE Gel preparation kit (#P2012, New Cell & Molecular Biotech., Suzhou, China). .. Separated protein bands were transferred to polyvinylidene fluoride (PVDF) membranes (#IPVH00010, Millipore, Boston, USA), which were then blocked with skim milk (#VIC141, VICMED, Xuzhou, China) by gentle shaking at room temperature for 1 h. The PVDF membranes were incubated overnight at 4 °C with the following primary antibodies: MSN polyclonal antibody (1:1000; #16495-1-AP, Proteintech, Wuhan, China), CD44 polyclonal antibody (recognizes the standard isoform of human CD44, 1:1000; #15675-1-AP, Proteintech, Wuhan, China), DNA-PKcs polyclonal antibody (1:1000; #19983-1-AP, Proteintech, Wuhan, China), KU70 polyclonal antibody (1:1000; #10723-1-AP, Proteintech, Wuhan, China), KU80 polyclonal antibody (1:1000; #16389-1-AP, Proteintech, Wuhan, China), AKT1 monoclonal antibody(2C5D1) (1:1000; #60203-2-Ig, Proteintech, Wuhan, China), phospho-AKT1 (Ser473) recombinant antibody(2E17) (1:1000; #80455-1-RR, Proteintech, Wuhan, China), and GAPDH monoclonal antibody(1E6D9) (1:1000; #60004-1- Ig, Proteintech, Wuhan, China). .. 12 h later, the PVDF membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (1:10000; #VSE12 and #VSE13, VICMED, Xuzhou, China) at room temperature for 1 h. After washing with washing buffer for 3 times, protein bands were visualized using an ECL chemiluminescence kit (#P10300, New Cell & Molecular Biotech., Suzhou, China) and a chemiluminescence imaging system (Tanon, Shanghai, China).



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    Image Search Results


    Journal: iScience

    Article Title: Manganese enhances DNA- or RNA-mediated innate immune response by inducing phosphorylation of TANK-binding kinase 1

    doi: 10.1016/j.isci.2022.105352

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-Ku80 , Cell Signaling Technology , Cat# 2753; RRID: AB_2257526.

    Techniques: Virus, Recombinant, Transformation Assay, Negative Control, Plasmid Preparation, Software